How to Reconstitute Peptides: Complete 2026 Guide

Reconstituting peptides is a critical step that determines dosing accuracy and compound stability. This guide covers everything from choosing your solvent to calculating exact injection volumes.

What You Need

• Lyophilized peptide vial (check mg amount on label)
• Bacteriostatic water (BAC water with 0.9% benzyl alcohol)
• Alcohol swabs for sterilization
• Insulin syringes (29-31 gauge)

Step-by-Step Process

1. Clean the vial tops with alcohol swabs and let dry
2. Draw your chosen volume of BAC water (typically 1-2 mL)
3. Inject slowly against the vial wall - never spray directly onto the powder
4. Gently swirl (do NOT shake) until fully dissolved
5. Store reconstituted peptide at 2-8C (refrigerator)

Calculating Your Dose

Use the formula: Volume (mL) = Desired dose (mcg) / Concentration (mcg/mL)

Example: 5mg vial + 2mL BAC water = 2500 mcg/mL. For a 250mcg dose: 250/2500 = 0.1 mL (10 units on insulin syringe).

Use our free reconstitution calculator for instant calculations.

Storage Guidelines

• Lyophilized (powder): Room temperature, away from light
• Reconstituted: Refrigerated (2-8C) for up to 30 days
• Never freeze reconstituted peptides

Common Mistakes

• Shaking the vial (denatures the peptide)
• Using sterile water instead of BAC water for multi-dose vials
• Not refrigerating after reconstitution
• Contaminating the rubber stopper