Reconstitution math question for first vial

Posted by aspiring_trailrun in Beginner Questions - 24 points, 7 comments.

Hey everyone. New here and still working through the basics. I have my first vial of BPC-157 coming and I want to make sure I get the reconstitution right before I even open it.

The vial says 5 mg and I plan to use bac water. 5 mg per ml which is 2500 mcg per ml. 1 ml on an insulin syringe.

That math seems right but I keep second guessing myself because the units flip between mg and mcg. Also not sure if 2 ml is too much volume for a 5 mg vial or if 1 ml is more standard. I have read a few guides but they all use slightly different numbers and it makes me nervous.

For context I am 40s male, CPT background, mostly looking at this for knee tendon irritation from trail running. Not expecting miracles just want to be precise with the basics. Any confirmation on the math or thoughts on volume would help a lot. Thanks.

Comments

• blake_sleepnerd: Your math checks out to me. Five milligrams divided by two milliliters gives 2.5 milligrams per milliliter, which is 2500 micrograms per milliliter, so a 250 microgram dose corresponds to 0.1 milliliter on a U‑100 syringe. I also chose to use two milliliters in my first vial because using only one milliliter would have made each 250 microgram dose too small to measure accurately.
• busy_macros: That makes sense, thanks for confirming. I was leaning toward 2 ml myself since the smaller doses are easier to measure. Did you notice any difference in how it felt or stored compared to 1 ml? Also do you pull it into the syringe right after mixing or let it sit a bit first?
• aspiring_trailrun: Thanks for the reply. My vial has not actually arrived yet so I cannot speak to feel or storage. I was hoping to nail down the math before it shows up. Do you find 2 ml stays stable for the full month in the fridge? And do you wait a few minutes after mixing before drawing or go straight in?
• aspiring_trailrun: Thanks for confirming the math. That is exactly what I needed. on the 1 ml volume making the 250 mcg dose harder to measure accurately. I was leaning toward 2 ml for that reason but kept seeing 1 ml in some guides. Did you run into any issues with the larger volume like site irritation or just more injections per vial?
• amber464: iverun 2 ml in a 5 mg vial a few times and it held fine for the full 30 days in the fridge imo. concentration ends up 2500 mcg per ml so 250 mcg is exactly 0.1 ml on an insulin syringe, math checks out. after mixing i let it sit five minutes, gentle swirl if needed, no shaking. draws clean after that. some people prefer 1 ml for less volume per shot but 2 ml makes low doses way easier to measure accurately.
• kim_sauna: Yeah, 2 mL is usually easier for tiny doses tbh, less squinting at the syringe like it’s a tax form 😅 I’ve not personally had site irritation from the extra volume, just make sure you’re gentle with the bac water and rotate spots. More volume per vial is fine, it just changes the concentration.
• aspiring_trailrun: Cheers, that makes sense. I was overthinking the 1 mL versus 2 mL thing, and your tax form line is bang on 😅 I think I’ll go with 2 mL for exactly that reason, easier maths on the tiny marks. Does rotating spots every time mean just alternating left and right, or do you mean moving around a bit more within the same general area?

Community discussion - research and educational context only. Not medical advice.