How do I reconstitute and dose Follistatin‑344 for my first 2‑week trial?

Posted by aspiring_codes in Beginner Questions - 1 points, 4 comments.

I’m new to peptides and just read about Follistatin‑344. The idea of blocking myostatin is tempting, but I have zero experience with reconstitution or dosing. The info I found says a typical dose is 100‑200 mcg daily for 10‑30 days, but the vial I have is 2 mg powder with bacteriostatic water recommended.

How much water should I add to get a workable concentration? Do I need to shake it, let it sit, or use a vortex? Once it’s in solution, what’s the best way to draw up a 150 mcg dose with an insulin syringe – do I calculate based on the total volume or is there a simpler trick? Also, any tips on rotating injection sites to avoid irritation would be appreciated.

I’m planning to do a short 2‑week run just to see if I notice any change in recovery after leg days, so I want to keep everything clean and safe. Thanks for any step‑by‑step guidance! 👀

Comments

• greg208: Not gonna lie i was in the same boat when i first tried follistatin‑344. I usually add 1 ml of bacteriostatic water to a 2 mg vial – that gives you a 2 µg/µl (2000 mcg/ml) stock, which is easy to work with. Give it a gentle swirl, don’t shake too hard or you’ll get bubbles, and let it sit a couple of minutes so the powder fully dissolves. To pull a 150 mcg dose you’d need 0.075 ml (75 µl) from that stock. Most insulin syringes are marked in units where 100 units = 1 ml, so 7.5 units is roughly
• liam_sleepnerd: Tbh that 1 ml makes a nice 2 µg/µl stock for me too, I just let it sit 3‑4 min after a gentle swirl. I usually pull the 150 mcg by marking 7.5 units on a 0.5 ml insulin syringe – 8 units works fine if you want a hair over. Have you noticed any injection site irritation with that volume?
• aspiring_codes: Thanks, that 1 ml to get a 2 µg/µl stock is exactly what I was aiming for. 5‑unit mark for the 150 µg dose. I haven’t seen any redness or soreness yet, but I’m only a day in. Do you have a favorite rotation pattern for sub‑Q on the thighs vs abdomen to keep it painless?
• aspiring_codes: Got it, 1 ml for a 2 mg vial sounds simple enough. 5 ml to keep the volume smaller, but your 2 µg/µl stock makes the math clean so I’ll go with the full 1 ml. I’ll use the gentle swirl and let it sit a minute – I was a bit nervous about bubbles. I’ll aim for 7–8 units on the insulin syringe for the 150 mcg dose. For site rotation, I’ve been alternating quadriceps and hamstring spots; do you find any particular area less prone to bruising?

Community discussion - research and educational context only. Not medical advice.